Journal: FEMS Microbiology Letters

Article Title: Attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment

doi: 10.1016/0378-1097(96)00243-1

Figure Lengend Snippet: Fig. 2. Attachment of bacteria to SAMs formed of o-substituted alkanethiolates on gold. (A) Staphylococcus epidermidis (ATCC 14990). (B) Deleya marina (ATCC 25374). For all surfaces, dura- tion of attachment was 2 h at a flow rate of 1.5 mUmin. Each data point represents the average f standard error of 10 samples. For each sample, 68 fields of view were averaged. Surfaces are arranged in order of increasing hydrophilicity.

Article Snippet: Bacterial strains The type strains of Staphylococcus epidermidis (ATCC 14990) and Deleya marina (ATCC 25374) were resuscitated from lyophilized stocks (American Type Culture Collection, Bethesda, MD) and stored at -70°C as a suspension in 20% glycerol in nutrient broth (S. epidermidis) or marine broth 2216 (D. marina).

Techniques: Bacteria

Journal: International Journal of Molecular Sciences

Article Title: Activity of Silver Nanoparticles against Staphylococcus spp.

doi: 10.3390/ijms23084298

Figure Lengend Snippet: Silver nanoparticles synthesized with compounds of various origins used against staphylococci.

Article Snippet: Silver-decorated submicron-sized biodegradable poly (DL-lactide-coglycolide) nanoparticles (Ag PLGA nanoparticles) , 299.6 , S. epidermidis ATCC 14990T , 38.5% viability of bacteria after treatment with Ag PLGA nanoparticles; viable colony counts for the biofilm decreased from 6.4 × 10 7 CFU/mL to 1.4 × 10 7 CFU/mL (more than four orders of magnitude); viable colony count for the bacterial cells after various treatment for 2 h decreased from 6.4 × 10 6 CFU/mL to 3.7 CFU/mL (1.7 million times less) , [ ] .

Techniques: Synthesized, Disruption, Derivative Assay, Modification, Produced, Inhibition, Incubation, Concentration Assay, Activity Assay, Comparison, Bacteria

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Minimum inhibitory concentration (MIC) of diethyldithiocarbamate (DDC), metal ions and the combination of both against S. epidermidis ATCC 35984.

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Minimal inhibitory concentration of the antibiotics methicillin (Meth), ceftazidime (Ceft), ciprofloxacin (Cip), vancomycin (Van), doxycycline (Doxy), amikacin (Amik), erythromycin (Erythro) and the compounds diethyldithiocarbamate (DDC) and Cu 2+ towards planktonic S. aureus , MRSA, S. epidermidis , E. coli and P. aeruginosa.

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Effect of diethyldithiocarbamate (DDC) and Cu 2+ concentrations (in μg/ml) on the viability of (A) MRSA Mu50, (B) MRSA 2, (C) Staphylococcus epidermidis ATCC 35984 and (D) S. epidermidis ATCC 14990 biofilms compared to monotherapy with Cu 2+ ( n = 3; * p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Synergistic effects of diethyldithiocarbamate in combination with Cu 2+ against S. aureus, MRSA and S. epidermidis biofilms.

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Comparison of stained MRSA Mu50 and S. epidermidis ATCC 35984 biofilms with LIVE/DEAD BacLight staining after treatment with 8 μg/ml diethyldithiocarbamate and 32 μg/ml Cu 2+ (DDC-Cu 2+ ). Confocal microscopy images results: green = viable bacteria; red = dead bacteria. (A) Untreated S. epidermidis ATCC 35984 biofilm at 20×. S. epidermidis ATCC 35984 biofilm after treatment with DDC-Cu 2+ at (B) 20× and (D) 100×. (C) Quantification of images as green/red ratio of untreated control (black) and treatment with DDC-Cu 2+ (grey) of MRSA and S. epidermidis ATCC 35984 biofilms ( n = 3–8; *** p < 0.001).

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques: Comparison, Staining, Confocal Microscopy, Bacteria, Control

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Effect of 8 μg/ml diethyldithiocarbamate (DDC; orange), 32 μg/ml Cu 2+ (blue) and combined DDC-Cu 2+ (grey) on (A) the cell index of MRSA Mu50 and (C) S. epidermidis ATCC 35984 over 48 h compared to the untreated control (black). Comparison of the mean cell index between 12 and 48 h for each treatment of (B) MRSA Mu50 and (D) S. epidermidis ATCC 35984 ( n > 3; *** p < 0.001).

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques: Control, Comparison

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Effect of diethyldithiocarbamate [DDC; orange; 8 μg/ml (A) , 6.4 mg/kg (B–D) ], Cu 2+ [blue; 32 μg/ml (A) , 25.6 mg/kg (B–D) ] and DDC-Cu 2+ (grey) on (A) fibroblast viability ( n = 3), on (B) probability of Galleria mellonella survival (30/group; n = 120), on the probability of survival of Galleria mellonella infected with (C) MRSA Mu50 (30/group; n = 120), and (D) infected with S. epidermidis ATCC 35984 (30/group; n = 120; NS = not significant; * p < 0.05; *** p < 0.001).

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques: Infection

Journal: Frontiers in Microbiology

Article Title: The combination of diethyldithiocarbamate and copper ions is active against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro and in vivo

doi: 10.3389/fmicb.2022.999893

Figure Lengend Snippet: Putative diethyldithiocarbamate and copper (DDC-Cu 2+ ) mode of action against S. aureus and S. epidermidis . (A) The antibacterial activity of Cu 2+ is limited by copper resistance mechanisms of bacteria. (B) The Cu(DDC) 2 complex inhibits the bacterial copper resistance mechanism but does not kill bacteria. (C) The combination of Cu(DDC) 2 complex and excessive Cu 2+ (called DDC-Cu 2+ ) effectively kills bacteria, as the Cu(DDC) 2 complex inhibits the copper resistance mechanisms, allowing for the excess Cu 2+ to increase copper induced toxicity.

Article Snippet: Synergistic and additive effects of DDC and Cu 2+ were observed against all planktonic MRSA (ΣFICi: MRSA Mu50 = 0.14; MRSA 1 = 0.67; MRSA 2 = 0.19; MRSA 3 = 0.88) and S. epidermidis strains (ΣFICi: S. epidermidis ATCC 14990 = 0.93; S. epidermidis ATCC 35984 = 0.63), except for S. aureus ATCC 25923 (ΣFICi = 1.23; ).

Techniques: Activity Assay, Bacteria